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- Dictionarycom·plete/kəmˈplēt/
adjective
- 1. having all the necessary or appropriate parts: "a complete list of courses offered by the college"
- 2. (often used for emphasis) to the greatest extent or degree; total: "a complete ban on smoking" Similar Opposite
verb
- 1. finish making or doing: "he completed his Ph.D. in 1983" Similar Opposite
- 2. make (something) whole or perfect: "he only needed one thing to complete his happiness" Similar
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cds means coding sequence as you can see in your file under features - source - it says "genomic DNA" and it contains the intron 347 - 660 (see mRNA in the file and CDS) as well as 5' and 3' UTR which cDNA wouldn't. -kylvalda-. Go to the forum page Printer Friendly Version.
Jul 9, 2008 · Besides helping verify your FACS histograms, the positive controls can tell you are the percentage of cells that are cycling. Normally HeLa and healthy tumor cell lines will all be cycling in pre-confluence growth conditions, but it should be checked. If after 18-24hr, nocodazole causes complete 4N arrest, but your drug doesn't, you can at ...
Jan 31, 2009 · Reverse Complement. Reverse Complement converts a DNA sequence into its reverse, complement, or reverse-complement counterpart. The entire IUPAC DNA alphabet is supported, and the case of each input sequence character is maintained. You may want to work with the reverse-complement of a sequence if it contains an ORF on the reverse strand.
Oct 18, 2006 · Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes ...
Oche. -oche-. 1. A strain is a bacterium that has some level of genetic definition, often plasmids that confer specific traits. An isolate, by contrast does not necessarily have such definition, but is simply the bugs that were isolated from the sample. 2.
Protocols. Complete PCR Guide (Laboratory of Dr. Bart Frank, Oklahoma Medical Research Foundation) In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond to those on synthetic oligonucleotide primers which are used to initiate the reaction.
Cellular ELISA Protocol. Add 100 æl/cell to 96 well culture plates. Blot dry. Dissolve in 900 ml di-H 2 O. Check pH and adjust to 9.6 necessary. Qs. to 1 liter. Dissolve in 900 ml di-H 2 O. Check pH and adjust to 6.0 if necessary. Qs. to 1 liter.
Vehicle treated is control. Vehicle is the same solvent in which your test reagent is dissolved.. could be aqueous or organic: water, saline, ethanol, dmso etc. so if you have your test compound A dissolved in PBS, and you add 100 ul of it to your 10 cm plate of cells, then, yo ualso have another control plate where you just add 100 ul of PBS.
Thanks in advance. Passage, split and subcultivation are all synonyms. The ratio is based on the initial amount of cells you had, so if you had a flask of cells and lifted the whole flask for splitting and suspended in 10 ml, threw away 5 ml and seeded two new flasks with 2.5 ml each you ratio would be 1:4 (i.e. you used a quarter of your cells ...
Aug 10, 2006 · Place the gel on top of the chromatography papers and cover the gel with the PVDF membrane. Put the remaining 3-mm stack of chromatography papers soaked with electrode buffer on top. Place the anode on top. Place a 5-kg load on top of the anode to avoid expansion of the gel during protein transfer.
