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The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
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PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query.
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What is BLAST? The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
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Awash in a sea of data, how do scientists identify the function of a newly cloned gene? Online resources like the Basic Local Alignment Search Tool (BLAST) provide a helping hand.
BLAST is one of the most widely used bioinformatics programs for sequence searching. [4] It addresses a fundamental problem in bioinformatics research. The heuristic algorithm it uses is much faster than other approaches, such as calculating an optimal alignment.
The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses).
Mar 17, 2014 · BLAST for beginners introduces students to blastn, a commonly used tool for comparing nucleotide sequences (DNA and RNA). This popular tutorial shows how to do a blast search with a nucleotide sequence, highlights information in the search results, and shows how to interpret the E value and alignment scores.
