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  1. MODULE 1: PLATELET COUNT AND PLATELET FUNCTION TESTS. Topic Outline: I. CLOT RETRACTION a) Standard Procedure b) Interpretation of results c) Other Methods for Clot Retraction d) Conditions that affect clot retraction. Learning Outcomes: Discuss the principle of Clot retraction Test. Perform Clot Retraction.

  2. Jun 5, 2013 · According to Dameshek, true polycythemia (polycythemia vera: PV) is a chronic myeloproliferative disorder of the total bone marrow without any evidence of invasiveness, in which erythrocytosis, leukocytosis, and thrombocytosis are all simultaneously present.

  3. 2. Dameshek Method or Wet Method. Materials: Microscopic slide Pipette Pipette shaker Rees Ecker fluid o Brilliant Cresyl Blue (stain) 0 gm o 40% Formalin 0 mL preservative o Sodium Citrate 100 mL To prevent clumping of platelet. Procedure: Disinfect the puncture site.

  4. Modified Dameshek Method. Uses siliconized medicine dropper to dilute the blood; The wet mount (Wet Method), the RBCs tend to concentrate at the edges of the coverslip. So, you have to count the RBCs in the central area. This is done to avoid false-increase the ratio of platelets to RBCs. III.

  5. Sep 20, 2007 · In 1951, William Dameshek described the concept of ‘myeloproliferative disorders (MPDs)’ by grouping together chronic myelogenous leukemia (CML), polycythemia vera (PV), essential thrombocythemia...

  6. In 1951, William Dameshek described the concept of 'myeloproliferative disorders (MPDs)' by grouping together chronic myelogenous leukemia (CML), polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF) and erythroleukemia; he reasoned that a self-perpetuating trilineage m ….

  7. The indirect (wet smear) technic of Dameshek 13 was used. The normal range by this method is 400,000 to 800,000 platelets per cu. mm. Differential Platelet Counts. One of us (J. F. -G.) counted 400 platelets in an evenly spread portion of the stained blood film.